Innovation & Advantages

  • High-throughput single exosome (100,000+) analysis

    Simultaneous analysis of 100,000+ single exosome proteins in each sample allows you to discover low-abundance exosome subsets in your sample.

  • 200+ multiplex protein detection

    The multiplex immunoassay technology can be used to analyze the characteristics of 200+ protein components in a single exosome in real time, providing more comprehensive and accurate data analysis results.

  • <5 μl sample volume without purification

    Most body fluids only require a small amount of samples, and there is no need for prior exosome isolation and purification steps, which further saves time and experimental costs.

  • Single-Signal molecular detection

    High-sensitivity data results with detection resolution accurate to single exosome surface molecular proteins, which can identify low-abundance exosome surface proteins with higher disease diagnostic value.

  • Suitable for almost all body fluids and culture fluids

    Such as plasma, urine, cell culture medium, and organ lavage fluid, which can be used for single exosome detection, and is suitable for a wide range of sample types, which greatly reduces the limitations of samples.

Proximity Barcoding Assay

The breakthrough single exosome detection technology has changed the dilemma that traditional exosome technology is often limited to total protein and total nucleic acid detection, and has realized high-precision and high-sensitivity exosome heterogeneity research.

Proximity Barcoding Assay (PBA) article: Wu et al. (2019) Profiling surface proteins on individual exosomes using a proximity barcoding assay

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Overview of PBA theory

The surface protein composition of single exosome in a sample can be converted into DNA sequence information by an antibody-DNA conjugate bound to it.

Process
Step 1:

The antibody-DNA conjugate recognizes and binds exosome surface proteins, and PBA technology can simultaneously recognize and label 200+ proteins on the surface of a single exosome.

Step 2:

Rolling cycle DNA sequences enable single exosome separation and labeling by recognizing single exosome surface markers.

Step 3:

Next-generation sequencing technology analyzes protein and exosome information in DNA sequences.

Step 4:

Bioinformatics analysis of single exosome proteomic information.

  • pba-img Multiplex antibody recognition
  • pba-img Single exosome proximity barcoding
  • pba-img High-throughput sequencing
  • pba-img Bioinformatics analysis

Reference

Reference